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anti igfbp2  (Proteintech)


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    Structured Review

    Proteintech anti igfbp2
    Anti Igfbp2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+igfbp2/IGFBP2+Antibody/10__1097_slash_js9__0000000000004351-139-23-24
    Average 94 stars, based on 31 article reviews
    anti igfbp2 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: IGFBP2 derived from PO-MSCs promote epithelial barrier destruction by activating FAK signaling in nasal polyps
    Article Snippet: Proteins were then transferred to polyvinylidene difluoride membranes (Millipore; cat. no. ISEQ00010, USA). .. The membranes were incubated with the following primary antibodies: anti-E-cadherin (CST, USA), anti-Vimentin (CST, USA), anti-N-cadherin (CST, USA), anti-TGF-β1(CST, USA), anti-ZO-1(Proteintech, China), anti-IGFBP2 (Proteintech, China), anti-Occludin (Proteintech, China), anti-Claudin-1 (Proteintech, China), anti-FAK (CST, USA), anti-MEK1/2 (CST, USA), and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Proteintech, China). ..

    Article Title: Diagnostic model and identification of IGFBP2 as an aging-related biomarker and therapeutic target for systemic sclerosis-associated interstitial lung disease through integrated bioinformatics and machine learning approaches
    Article Snippet: .. After blocking with 5% BSA for 1 h at room temperature, the sections were incubated overnight at 4°C with the following primary antibodies: anti-IGFBP2 (Proteintech Cat#11 065-3-AP, RRID: AB_2123101), anti-keratin 5 (Proteintech Cat# 66 727-1-Ig, RRID: AB_2882077), anti-p16 (Beyotime,#AF1069,RRID:N/ A), Anti-Histone H2AX (ZenBio Cat# 201 082-2A9, RRID: AB_2722722), anti-Collagen I (Proteintech Cat #66761-1-Ig, RRID: AB_2882107), anti-CD31 (Proteintech Cat #28083- 1-AP, RRID: AB_ 2 881 055), and anti-SFTPC (Proteintech Cat #10774-1-AP, RRID: AB_2185497). .. Subsequently, fluorophore-conjugated secondary antibodies were applied for 2 h at room temperature, followed by nuclear counterstaining with 4’,6-diamidino-2-phenylindole (DAPI; Beyotime,#C1005, RRID: N/A).

    Article Title: Identification and Verification of Key Genes Associated with Temozolomide Resistance in Glioblastoma Based on Comprehensive Bioinformatics Analysis
    Article Snippet: .. The membranes were blocked with 5% non-fat dry milk in Tris-buffered saline with Tween-20 (TBST) for 1 hour at room temperature and then incubated with the following primary antibodies at 4°C overnight: anti-IGFBP2 (1:1000; Cat No. 11065-3-AP, Proteintech), anti-PITX1 (1:1000; Cat No. 10873-1-AP, Proteintech), and anti-TNFRSF11B (1:3000; Cat No. PS-18571, Ab-mart). ..

    Article Title: Exosomes from cisplatin-induced dormant cancer cells facilitate the formation of premetastatic niche in bone marrow through activating glycolysis of BMSCs
    Article Snippet: .. The membranes were blocked with 5% bovine serum albumin (BSA) for 1 h, detected for anti-HSP70 (EXOAB-Hsp70A-1, System Biosciences, USA), anti-CD63 (EXOAB-CD63A-1, System Biosciences, USA), anti-TSG101 (28283-1-AP, Proteintech, USA), anti-IGFBP2 (11065-3-AP, Proteintech, USA), anti-IGF-2 (ab177467, Abcam, UK), anti-IGF-1R (9750T, Cell Signaling Technology, USA), and anti-p-IGF-1R (Tyr1135, 3918T, Cell Signaling Technology, USA) at 4°C overnight, and then incubated with anti-rabbit IgG conjugated with horseradish peroxidase (HRP) (SA00001-2, Proteintech, USA) for 1 h at room temperature. .. The Infrared Imaging System (LI-COR Biosciences, Lincoln, NE, USA) was used to scan and analyze the images.

    Blocking Assay:

    Article Title: Diagnostic model and identification of IGFBP2 as an aging-related biomarker and therapeutic target for systemic sclerosis-associated interstitial lung disease through integrated bioinformatics and machine learning approaches
    Article Snippet: .. After blocking with 5% BSA for 1 h at room temperature, the sections were incubated overnight at 4°C with the following primary antibodies: anti-IGFBP2 (Proteintech Cat#11 065-3-AP, RRID: AB_2123101), anti-keratin 5 (Proteintech Cat# 66 727-1-Ig, RRID: AB_2882077), anti-p16 (Beyotime,#AF1069,RRID:N/ A), Anti-Histone H2AX (ZenBio Cat# 201 082-2A9, RRID: AB_2722722), anti-Collagen I (Proteintech Cat #66761-1-Ig, RRID: AB_2882107), anti-CD31 (Proteintech Cat #28083- 1-AP, RRID: AB_ 2 881 055), and anti-SFTPC (Proteintech Cat #10774-1-AP, RRID: AB_2185497). .. Subsequently, fluorophore-conjugated secondary antibodies were applied for 2 h at room temperature, followed by nuclear counterstaining with 4’,6-diamidino-2-phenylindole (DAPI; Beyotime,#C1005, RRID: N/A).

    Saline:

    Article Title: Identification and Verification of Key Genes Associated with Temozolomide Resistance in Glioblastoma Based on Comprehensive Bioinformatics Analysis
    Article Snippet: .. The membranes were blocked with 5% non-fat dry milk in Tris-buffered saline with Tween-20 (TBST) for 1 hour at room temperature and then incubated with the following primary antibodies at 4°C overnight: anti-IGFBP2 (1:1000; Cat No. 11065-3-AP, Proteintech), anti-PITX1 (1:1000; Cat No. 10873-1-AP, Proteintech), and anti-TNFRSF11B (1:3000; Cat No. PS-18571, Ab-mart). ..



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    Proteintech ccnd1
    a Schematic of the coinjection model of D10 LU-RAS (vehicle), LU-RAS/KD-WT, or LU-RAS/KD-AA cells with A549 cells in nude mice. b , c Fluorescence images of GFP-expressing A549 cells injected alone or coinjected with LU-RAS (vehicle), LU-RAS/KD-WT, or LU-RAS/KD-AA cells (NIR790-stained) at D10 of OIS ( n = 6 per group); images were acquired on days 5, 15, and 28. One representative image is shown ( b ). The relative fluorescence intensity was quantified ( c ). d Tumors were excised after 28 days. e Tumor volumes were measured. f Immunoblot analysis of senescence markers in xenograft tumor samples. n = 3 biologically independent samples were analyzed, and similar results were obtained across replicates. g , h Xenograft tumor samples were subjected to H&E, SA-β-Gal, IL6, IGFBP2, <t>CCND1,</t> and STAG2 staining. Scale bars, 100 μm. Representative images ( g ); quantitative analysis of mean IHC staining ( h ). The data are presented as mean ± SD from six biological replicates with similar results. p values were calculated using a two-tailed unpaired Student’s t -test ( c , e , h ). The source data for the graphs in ( c , e , h ) and immunoblots in ( f ) are provided in the File.
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    Image Search Results


    a Schematic of the coinjection model of D10 LU-RAS (vehicle), LU-RAS/KD-WT, or LU-RAS/KD-AA cells with A549 cells in nude mice. b , c Fluorescence images of GFP-expressing A549 cells injected alone or coinjected with LU-RAS (vehicle), LU-RAS/KD-WT, or LU-RAS/KD-AA cells (NIR790-stained) at D10 of OIS ( n = 6 per group); images were acquired on days 5, 15, and 28. One representative image is shown ( b ). The relative fluorescence intensity was quantified ( c ). d Tumors were excised after 28 days. e Tumor volumes were measured. f Immunoblot analysis of senescence markers in xenograft tumor samples. n = 3 biologically independent samples were analyzed, and similar results were obtained across replicates. g , h Xenograft tumor samples were subjected to H&E, SA-β-Gal, IL6, IGFBP2, CCND1, and STAG2 staining. Scale bars, 100 μm. Representative images ( g ); quantitative analysis of mean IHC staining ( h ). The data are presented as mean ± SD from six biological replicates with similar results. p values were calculated using a two-tailed unpaired Student’s t -test ( c , e , h ). The source data for the graphs in ( c , e , h ) and immunoblots in ( f ) are provided in the File.

    Journal: Nature Communications

    Article Title: Time-resolved multiomics profiling reveals chromatin O-GlcNAc modification promotes senescence-associated transcriptional program

    doi: 10.1038/s41467-025-68143-z

    Figure Lengend Snippet: a Schematic of the coinjection model of D10 LU-RAS (vehicle), LU-RAS/KD-WT, or LU-RAS/KD-AA cells with A549 cells in nude mice. b , c Fluorescence images of GFP-expressing A549 cells injected alone or coinjected with LU-RAS (vehicle), LU-RAS/KD-WT, or LU-RAS/KD-AA cells (NIR790-stained) at D10 of OIS ( n = 6 per group); images were acquired on days 5, 15, and 28. One representative image is shown ( b ). The relative fluorescence intensity was quantified ( c ). d Tumors were excised after 28 days. e Tumor volumes were measured. f Immunoblot analysis of senescence markers in xenograft tumor samples. n = 3 biologically independent samples were analyzed, and similar results were obtained across replicates. g , h Xenograft tumor samples were subjected to H&E, SA-β-Gal, IL6, IGFBP2, CCND1, and STAG2 staining. Scale bars, 100 μm. Representative images ( g ); quantitative analysis of mean IHC staining ( h ). The data are presented as mean ± SD from six biological replicates with similar results. p values were calculated using a two-tailed unpaired Student’s t -test ( c , e , h ). The source data for the graphs in ( c , e , h ) and immunoblots in ( f ) are provided in the File.

    Article Snippet: After the experimental endpoint, mice were euthanized, and tumors were excised, fixed, and subjected to sectioning and staining procedures, including H&E staining, SA-β-Gal staining, and immunostaining with antibodies for IL-6 (Proteintech, #21865-1-AP, 1:100), IGFBP2 (Proteintech, #11065-3-AP, 1:100), CCND1 (Proteintech, # 26939-1-AP, 1:1000), and STAG2 (Proteintech, #19837-1-AP, 1:100).

    Techniques: Fluorescence, Expressing, Injection, Staining, Western Blot, Immunohistochemistry, Two Tailed Test